Journal of Experimental Botany 52: 1753-1759
(2001)
Antisense-transformation reveals novel roles for class I ß-1,3-glucanase
in tobacco seed after-ripening and photodormancy
| Lines a | % Endosperm rupture in continuous light b | % Non-photodormancy c (dark-germination) | |||
| ‘Fresh’ seed | Afterripened seed | ‘Fresh’ seed | Afterripened seed | ||
| Wild type | 49.8 ± 5.2 | 79.2 ± 6.8 | 0.4 ± 0.2 | 50.3 ± 16.5 (0.0 - 80.6) | |
| TCIB1-2 | 49.7 ±2.1 | 81.4 ± 5.2 | 0.3 ± 0.2 | 40.0 ± 12.6 (9.1 - 80.1) | |
| TKSG7-32 | 74.4 ± 2.1 | 79.0 ± 2.4 | 0.3 ± 0.3 | 29.7 ± 13.5 (3.0 - 63.6) | |
| TKSG7-38 | 77.1 ± 2.1 | 73.5 ± 0.1 | 0.9 ± 0.2 | 39.0 ± 18.9 (0.0 - 82.4) | |
| TKSG7-43 | 75.3 ± 3.6 | 75.8 ± 7.4 | 0.4 ± 0.2 | 43.6 ± 19.6 (9.4 - 90.2) | |
| TGAG2-24 | 48.8 ± 5.4 | 15.8 ± 4.5 | 0.2 ± 0.1 | 7.8 ± 2.6 (3.0 - 15.5) | |
| TGAG2-19 | --- d | 9.4 ± 0.7 | --- | 1.8 ± 0.7 (0.0 - 2.9) | |
| TKAG4-31 | 47.3 ± 6.2 | 4.9 ± 1.7 | 0.6 ± 0.3 | 3.2 ± 2.3 (0.0 - 10.2) | |
| TKAG4-50 | --- | 11.8 ± 2.1 | --- | 5.6 ± 1.5 (1.8 - 9.6) | |
 |
|
|
|
|
|
a Wild type and independent, homozygous, monogenic vector-control (TCIB1), sense ßGLU I (TKSG7) and antisense ßGLU I (TGAG2, TKAG4) tobacco lines selected from the S2 seed generation. Data for sense seeds obtained in the same experiment is from Leubner-Metzger and Meins (2000).
b Mean ± SE of ‘fresh’ (directly after harvest) and afterripened (≥ 6 months of dry storage) seed from 3-6 capsules scored 72 h and 67 h after the start of imbibition in continuous light in control medium without antibiotics, respectively.
c Mean ± SE of ‘fresh’ and afterripened seed from 3-6 capsules scored after 10 days incubation in the dark in control medium without antibiotics. The range obtained with different capsules is shown in parenthesis.
d Not determined.
| Article in PDF format (160 KB) | Abstract Fig. 1 Fig. 2 Table 1 Table 2 Glucanase constructs |
 ©
Society for Experimental Biologyhttp://jxb.oupjournals.org/ |
|
|
The Seed Biology Place |
Webdesign Gerhard Leubner 2000 |
