Plant Journal 23: 215-221 (2000)
Sense transformation reveals a novel role for class I ß-1,3-glucanase
in tobacco seed germination
Received: 21 March 2000 / Accepted: 5 April 2000
| Continuous light | Darkness | |||||||
| Endosperm rupture (%) (b) | ßGLU (pkat / seed) (c) | Non-photodormancy (%) (d) | ||||||
| Lines (a) |
‘Fresh’ seed |
After-ripened seed |
‘Fresh’ seed |
After-ripened seed |
‘Fresh’ seed |
After-ripened seed |
||
| Wild type | 49.8 ± 5.2 | 79.2 ± 6.8 | 2.4 ± 0.1 | 3.2 ± 0.2 | 0.4 ± 0.2 | 50.3 ± 16.5 (0.0 - 80.6) | ||
| TCIB1-2 | 49.7 ± 2.1 | 81.4 ± 5.2 | 2.2 ± 0.1 | 2.9 ± 0.2 | 0.3 ± 0.2 | 40.0 ± 12.6 (9.1 - 80.1) | ||
| TKSG7-32 | 74.4 ± 2.1 | 79.0 ± 2.4 | 9.1 ± 0.2 | 5.3 ± 0.2 | 0.3 ± 0.3 | 29.7 ± 13.5 (3.0 - 63.6) | ||
| TKSG7-38 | 77.1 ± 2.1 | 73.5 ± 0.1 | 8.9 ± 0.2 | 4.9 ± 0.3 | 0.9 ± 0.2 | 39.0 ± 18.9 (0.0 - 82.4) | ||
| TKSG7-43 | 75.3 ± 3.6 | 75.8 ± 7.4 | 8.2 ± 0.5 | 4.7 ± 0.4 | 0.4 ± 0.2 | 43.6 ± 19.6 (9.4 - 90.2) | ||
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a Wild type and independent, homozygous, monogenic vector-control (TCIB1) and sense ßGLU I (TKSG7) tobacco lines selected from the S2 seed generation.
b Mean ± SE of 'fresh' (directly after harvest) and afterripened (≥ 6 months of dry storage) seed from 3-6 capsules scored 72 h and 67 h, respectively, after the start of imbibition in continuous light in control medium without antibiotics.
c Mean ± SE ßGLU activities of protein extracts prepared from seed samples.
d Mean ± SE of 'fresh' and afterripened seed from 3-6 capsules scored after 10 days incubation in the dark in control medium without antibiotics. The range obtained with different capsules is shown in parenthesis.
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