Planta 199: 282-288 (1999)

Effects of gibberellins, darkness and osmotica on endosperm rupture and class I ß-1,3-glucanase induction in tobacco seed germination

Gerhard Leubner-Metzger, Corinne Fründt, Frederick Meins, Jr.
GA and ABA tobacco seed germination Fig. 1A-E. The effect of GA4 and ABA treatment on the time course of tobacco seed germination and ß-1,3-glucanase activity in seeds incubated in continuous light (A-C) or in the dark (D-F).

The incidence of seed coat rupture (A and D) and endosperm rupture (B and E) expressed in per cent of 100-200 seeds scored. The activity of ß-1,3-glucanase (C and F) expressed in pkat/(µg protein). Seeds were incubated in the absence (control) or presence of 10-5 M GA4, 10-5 M ABA, or GA4 and ABA added to the medium at the start of the experiment. The data are presented as mean values, usually for at least two samples in two independent experiments. Error bars= maximum ±SE

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