Journal of Experimental Botany 62: 5131–5147 (2011)

Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]

Antje Voegele, Ada Linkies, Kerstin Müller*, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada

Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214

Supplemental Table S2. cDNA clones obtained of the early micropylar endosperm SSH library.
Putative gene function was based on the highest BLAST hit, usually Arabidopsis if not indicated otherwise. Based on the data of Ogawa et al. (2003) and Preston et al. (2009) GA-induction during seed germination of A. thaliana Ler was determined by eNorthern (Toufighi et al., 2005) using the BAR website (http://bar.utoronto.ca/). Transcripts with a ratio of at least 1.5 between GA and control treatments at least one ot the three time points (3h, 6h, 9h) were considered as GA-induced ("Yes").

Clone number

GenBank
accession number

Locus of closest
Arabidopsishomologue

Putative gene product / function

Sequence similarity to Arabidopsis (%) ,
if not indicated otherwise

GA-induciblity

pUC19-8h23

HO208984

At1g08110

lactoylgluthatione lyase / glyoxylase1

91.8

No

8h-151

HO208845

At1g08770

PRA1 (prenylated rab acceptor)

80.2

No

8h-106

HO208806

At1g11350

universal stress protein (USP)

87.4

Yes

8h-38

HO208897

At1g15690

AVP1 (vacuolar-type H+-pumping pyrophosphatease 1)

96.2

Yes

8h-93

HO208946

At1g17620

unknown protein

83.8

Yes

pTopo-8h19

HO208958

At1g17720

serine/threonine protein phosphatase

72.5

Yes

8h-17

HO208861

At1g18210

calcium-binding

87.9

No

8h-54

HO208913

At1g18540

60S ribosomal protein L6 (RPL6A)

84.8

No

8h-69

HO208923

At1g18540

60S ribosomal protein L6 (RPL6A)

90.6

No

8h-104

HO208804

At1g19770

PUP14 (purin permease)

86.8

No

8h-176

HO208864

At1g23010

multi-copper oxidase type I family

82.0

No

pTopo-8h13

HO208953

At1g30270

CIPK23 (CBL-interacting protein kinase )

94.4

Yes

8h-162

HO208854

At1g30690

SEC14 (cytosolic factor)

87.1

Yes

8h-31

HO208891

At1g31850

dehydration-responsive

91.7

No

8h-189

HO208876

At1g47128

cysteine proteinase RD21A

89.5

Yes

8h-56

HO208915

At1g36280

adenylosuccinate lyase

93.1

Yes

8h-158

HO208851

At1g53510

MPK18 (map kinase)

88.2

No

8h-187

HO208874

At1g56090

tetratricopeptide repeat (TPR)-containing protein

85.3

No

8h-52

HO208911

At1g56300

DNAJ heat shock N-terminal domain-containing

86.2

No

8h-141

HO208835

At1g60160

potassium transporter family protein

93.2

Yes

8h-111

HO208811

At1g63000

RHM1/ROL1 (rhamnose biosynthesis)

87.7

Yes

8h-149

HO208842

At1g63000

RHM1/ROL1 (rhamnose biosynthesis)

86.6

No

8h-44

HO208902

At1g65930

isocitrate dehydrogenase

93.4

No

8h-129

HO208824

At1g64650

unknown protein

87.6

No

8h-185

HO208872

At1g64650

unknown protein

87.5

No

pTopo-8h2

HO208959

At1g69910

protein kinase family protein

85.3

No

8h-2

HO208881

At1g70090

GATL9 (galacturonosyltransferase-like)

89.7

No

8h-130

HO208825

At1g71920

histidinol-phosphate aminotransferase

92.1

No

8h-91

HO208944

At1g72510

unknown protein

83.1

Yes

8h-182

HO208870

At1g79850

Ribosomal protein S17

77.2

No

8h-171

HO208862

At1g80120

unknown protein

86.2

No

pUC19-8h11

HO208975

At2g05220

40S ribosomal protein S17 (RPS17B)

85.3

No

pTopo-8h9

HO208970

At2g05755

integral membrane family protein

80.4

Yes

8h-16

HO208852

At2g05929

subtilisin serine protease

87.7

No

8h-96

HO208948

At2g16365

F-box family

85.9

No

8h-8

HO208932

At2g17420

NADH-dependent thioredoxin reductase 2

79.5

No

Puc19-19

HO208981

At2g17930

inositol or phosphatidylinositol kinase/ phosphotransferase

94.1

No

8h-48

HO208906

At2g22090

UBA1 (ubiquitin activating enzyme)

90.0

No

8h-160

HO208853

At2g22500

mitochondrial dicarboxylate carrier protein

73.7

Yes

8h-18

HO208867

At2g23310

RERC1C1 (endoplasmatic reticulum retrieval 1C)

79.1

No

8h-163

HO208855

At2g24280

serine carboxypeptidase S28 family

89.0

No

8h-153

HO208847

At2g28190

CSD2 (copper/zinc SOD)

93.1

No

8h-29

HO208889

At2g32070

CCR4-NOT transcription complex protein

91.4

No

8h-135

HO208829

At2g32070

CCR4-NOT transcription complex protein

91.7

No

8h-9

HO208943

At2g34250

sec61 (protein transport)

95.4

No

8h-128

HO208823

At2g34480

60S ribosomal protein L18A

90.5

No

8h-51

HO208910

At2g36460

putative fructose biphosphate aldolase

88.2

No

8h-79

HO208931

At2g36460

putative fructose biphosphate aldolase

88.1

No

pTopo-8h23

HO208962

At2g36460

putative fructose biphosphate aldolase

88.0

No

pTopo-8h3

HO208964

At2g37640

EXPA3 (expansin)

76.5

Yes

pTopo-8h5

HO208966

At2g37640

EXPA3 (expansin)

76.5

No

pTopo-8h12

HO208952

At2g37640

EXPA3 (expansin)

81.8

No

8h-166

HO208857

At2g38710

AMMECR1 family

91.6

No

8h-191

HO208879

At2g39290

PGPS 1 (phosphatidylglycerolphosphate synthase 2C)

91.4

No

8h-53

HO208912

At2g40000

putative nematode resistant

90.7

Yes

8h-119

HO208814

At2g40330

Bet vI allergen family; abscisic acid receptor PYL6

80.7

Yes

pUC19-8h22

HO208983

At2g41900

zinc-finger (CCCH-type) family

89.3

No

pTopo-8h21

HO208961

At2g42700

unknown protein

89.3

No

pTopo-8h24

HO208963

At2g42700

unknown protein

85.3

No

8h-71

HO208924

At2g43160

epsin N-terminal homology domain-containing

93.2

No

8h-45

HO208903

At2g46330

AGP16 (arabinogalactan protein 16)

80.1

Yes

8h-28

HO208888

At2g47640

small nuclear riboonucleoprotein D2

87.7

No

8h-72

HO208925

At2g47640

small nuclear riboonucleoprotein D2

86.9

No

8h-39

HO208898

At3g01360

unknown protein

85.6

No

8h-188

HO208875

At3g02880

leucine-rich repeat transmembrane protein kinase

78.9

Yes

pUC19-8h14

HO208978

At3g03570

expressed protein

91.1

Yes

8h-169

HO208860

At3g03640

Beta glucosidase 25

89.4

No

8h-137

HO208831

At3g03710

RIF10 (resistant to inhibition with FSM 10)

81.6

No

8h-165

HO208856

At3g05880

RCI2A (rare cold inducible)

77.2

No

pUC19-8h12

HO208976

At3g07390

plant IF-like protein

77.1

Yes

8h-19

HO208877

At3g08030

unknown protein

89.1

No

8h-150

HO208844

At3g09630

60S ribosomal protein L1

80.5

No

8h-192

HO208880

At3g10930

unknown protein

74.2

Yes

pTopo-8h7

HO208968

At3g10980

Senescence associated gene 20 SAG20

87.6

No

8h-122

HO208817

At3g11400

EIF3G (translation initiation factor)

88.8

No

8h-89

HO208942

At3g11510

40S ribosomal protein S14 (RPS14B)

87.7

No

8h-177

HO208865

At3g14090

unknown protein

87.4

No

Puc19-8h16

HO208979

At3g16270

unknown protein

71.6

No

8h-12

HO208815

At3g16640

TCTP (translationally controlled tumor)

80.3

No

8h-26

HO208886

At3g16640

TCTP (translationally controlled tumor)

88.1

No

8h-84

HO208937

At3g18190

chaperonin

83.1

No

8h-190

HO208878

At3g19920

unknown protein

85.3

No

8h-145

HO208838

At3g20898

unknown protein

83.1

No

Puc19-8h10

HO208974

At3g21720

isocitrate lyase

94.0 (B. napus)

No

8h-110

HO208810

At3g21890

zinc-finger (B-box type) family

74.0

Yes

8h-181

HO208869

At3g24830

60S ribosomal protein L13A (RPL13aB)

91.9

No

8h-143

HO208837

At3g25040

HDEL ER lumen protein

82.0

Yes

8h-105

HO208805

At3g45970

EXLA1 (expansin-like)

85.2

No

pTopo-8h14

HO208954

At3g48140

senescence-associated

88.9

Yes

8h-62

HO208917

At3g49720

unknown protein

89.6

Yes

8h-174

HO208863

At3g52580

40S ribosomal protein S14 (RPS14C)

87.8

No

8h-179

HO208866

At3g55440

triose-phosphate isomerase (TPI)

91.3

No

8h-146

HO208839

At3g57090

binding (BIGYIN)

84.3

No

Puc19-8h7

HO208988

At3g57520

putative imbibition protein homolog

91.3

Yes

8h-64

HO208972

At3g57550

AGK2 (guanylate-kinase)

91.1

Yes

8h-99

HO208949

At3g59770

SAC 9 (suppressor of actin)

90.4

No

Puc19-8h20

HO208982

At3g63030

MBD4 (mathyl-CpG-binding domain 4, DNA-binding)

83.2

No

8h-107

HO208807

At4g00730

transcription factor/ transcription regulator (Anthocyanless 2)

74.1

No

8h-76

HO208929

At4g05050

UBQ11 (ubiquitin)

88.2

Yes

pTopo-8h15

HO208955

At4g11740

SAY1 (Isolated as a suppressor of a dominant mutant in the Ara4 gene )

80.8

No

8h-136

HO208830

At4g13010

oxidoreductase, zinc-binding dehydrogenase family

85.2

No

8h-133

HO208827

At4g13940

HOG1 (homology-dependent gene silencing, adenosylhomocysteinase)

93.6

No

8h-67

HO208921

At4g15470

unknown protein

80.7

No

pUC19-8h18

HO208980

At4g15470

unknown protein

90.8

No

8h-35

HO208894

At4g15530

PPDK (pyruvate orthophosphate dikinase)

92.9

Yes

8h-78

HO208930

At4g16563

aspartyl protease family

87.4

No

8h-127

HO208822

At4g17420

unknown protein

92.5

No

8h-27

HO208887

At4g18650

transcription-factor related

86.0

Yes

8h-87

HO208940

At4g20860

FAD-binding domain

90.0

No

8h-88

HO208941

At4g20860

FAD-binding domain

90.0

No

8h-102

HO208802

At4g22820

AN1-like zinc finger family

79.5

No

8h-156

HO208849

At4g24220

VEP1 (vein patterning)

94.1

No

8h-5

HO208908

At4g24770

RBP31 (RNA binding)

76.8

No

8h-74

HO208927

At4g26000

PEP (nucleic acid binding)

91.0

No

8h-101

HO208801

At4g27070

TSB2 (tryptophane synthase beta subunit)

82.5

No

pTopo-8h1

HO208950

At4g27320

universal stress protein (USP)

81.8

Yes

8h-6

HO208916

At4g27652

unknown protein

73.7

No

8h-138

HO208832

At4g28440

DNA-binding protein-related

90.1

No

8h-22

HO208884

At4g29130

HXK1 (hexokinase, glucose insensitive 1)

93.9

Yes

8h-75

HO208928

At4g29390

40S ribosomal protein S30 (RPS30B)

91.5

No

8h-36

HO208895

At4g30270

MERI5B (xyloglucan endo-1,4-Betaglucanase)

89.3

No

8h-21

HO208883

At4g30280

XTH18

86.9

Yes

8h-46

HO208904

At4g30280

XTH18

87.4

Yes

8h-47

HO208905

At4g30280

XTH18

87.4

Yes

8h-131

HO208826

At4g30280

XTH18

87.4

Yes

pTopo-8h6

HO208967

At4g30280

XTH18

85.7

Yes

8h-55

HO208914

At4g30290

XTH19

80.6

Yes

8h-25

HO208885

At4g31130

unknown protein

85.7

No

Puc19-8h1

HO208973

At4g32020

unknown protein

69.6

No

8h-73

HO208926

At4g32130

unknown protein

72.5

No

8h-86

HO208939

At4g32390

phosphate-translocator-related

90.4

No

8h-95

HO208947

At4g34555

40S ribosomal protein S25

81.8

Yes

8h-152

HO208846

At4g37580

HLS1 (hookless, N-acetyltransferase)

85.6

Yes

8h-34

HO208893

At4g38740

ROC1 (peptidylprolyl isomerase)

89.5

No

8h-20

HO208882

At4g38800

MTN1 (methylthioadenosin nucleosidase)

89.4

No

8h-83

HO208936

At5g01320

pyruvate decarboxylase

83.4

Yes

8h-167

HO208858

At5g01710

unknown protein

89.0

No

8h-139

HO208833

At5g02260

EXPA9 (expansin A9)

78.2

Yes

8h-184

HO208871

At5g02280

ATEXPA9 (ARABIDOPSIS THALIANA EXPANSIN A9) (ATEXPA9)

78.4

No

8h-49

HO208907

At5g02610

60S ribosomal protein L35 (RPL35D)

84.3

No

8h-50

HO208909

At5g02610

60S ribosomal protein L35 (RPL35D)

84.3

No

pTopo-8h17

HO208957

At5g03040

calmodulin binding (iqd2); (IQ-domain 2)

89.5

Yes

8h-164

HO208971

At5g03880

electron carrier

78.3

No

8h-40

HO208899

At5g07340

Calnexin

83.0

No

pTopo-8h20

HO208960

At5g07830

Beta-glucuronidase AtGUS2

91.2

No

8h-103

HO208803

At5g08160

PK3 serine/threonine protein kinase

93.2

No

Puc19-8h4

HO208987

At5g10750

unknown protein

87.6

No

8h-30

HO208890

At5g10770

chloroplast nucleoid DNA-binding protein

82.6

Yes

8h-157

HO208850

At5g10860

CBS domaine containing

84.1

No

8h-41

HO208900

At5g11090

serine-rich protein-related

81.2

No

8h-123

HO208818

At5g12140

CYS1 (cystatin)

83.4

No

8h-63

HO208918

At5g12850

zinc-finger (CCCH-type) family

89.6

Yes

8h-112

HO208812

At5g14450

GDSL-motif lipase/hydrolase family

90.1

Yes

8h-148

HO208841

At5g15650

RGP2 (reversibly glycosylated polypeptide-3)

90.8

Yes

pTopo-8h16

HO208956

At5g15850

COL1 (constance-like)

86.0

Yes

8h-147

HO208840

At5g17860

CAX7 (calcium exchanger)

89.3

Yes

8h-134

HO208828

At5g17920

CIMS (cobalamin-independent methionine synthase)

93.2

Yes

Puc19-8h9

HO208989

At5g20290

40S ribosomal protein S8 (RPS8A)

81.0

No

8h-168

HO208859

At5g25460

Unknown protein

93.9

No

8h-33

HO208892

At5g28060

40S ribosomal protein S24 (RPS24B)

78.9

No

8h-42

HO208901

At5g29560

Calcium-ion-binding

89.0

No

pTopo-8h4

HO208965

At5g35160

Unknown protein

85.2

No

8h-82

HO208935

At5g35460

unknown protein

89.9

No

8h-120

HO208816

At5g35560

MOK9_2

86.6

No

8h-85

HO208938

At5g38890

exoribonuclease-related

91.1

Yes

8h-116

HO208813

At5g43940

ADH2 alcohol dehydrogenase

92.2

No

Puc19-8h3

HO208986

At5g44420

PDF1.2c (plant defensin 1.2c) (PDF1.2c)

81.3

No

8h-68

HO208922

At5g45280

pectinacetylesterase

90.4

Yes

Puc19-8h24

HO208985

At5g45775

60S ribosomal protein L11 (RPL11D)

92.8

No

8h-65

HO208919

At5g48545

unknown protein

88.6

No

8h-125

HO208820

At5g51570

band 7 family protein

83.1

No

8h-180

HO208868

At5g53570

RabGAP/TBC domain-containing protein

84.1

No

8h-142

HO208836

At5g54855

pollen allergene Ole e1 and extensin family

90.2

No

8h-186

HO208873

At5g55190

RAN3 (RAN GTPase gene family)

93.0

No

pTopo-8h8

HO208969

At5g56030

HSP81-2 (early-responsive to dehydration)

91.2

Yes

8h-109

HO208809

At5g60390

elongation factor 1-alpha

85.6

No

8h-66

HO208920

At5g61230

Ankyrin repeat family

76.9

No

8h-124

HO208819

At5g61820

unknown protein

73.3

No

8h-108

HO208808

At5g64270

splicing factor

92.0

No

8h-155

HO208848

At5g64310

Arabinogalactan protein 1

69.9

Yes

8h-92

HO208945

At5g64400

unknown protein

82.7

No

8h-14

HO208834

At5g65140

trehalose-6-phosphate phosphatase

92.0

Yes

8h-15

HO208843

At5g66590

allergen V5/Tpx-1-related

84.4

No

8h-80

HO208933

At5g66590

allergen V5/Tpx-1-related

82.9

No


Article in PDF format (1.5 MB)
Supplementary data file (6.5 MB)
Abstract
Supplementary:
Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3       Fig. S4       Tab. S1       Tab. S2       Tab. S3
         
 
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