New Phytologist 184: 885-897 (2009)

The NADPH-Oxidase AtrbohB plays a role in Arabidopsis seed after-ripening [W]

Kerstin Müller, Anna Catharina Carstens, Ada Linkies, Miguel Angel Torres, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., A.C.C., A.L., G.L.-M.)
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón (Madrid), Spain (M.A.T.)

Received April 30, 2009; accepted July 13, 2009; published online September 15, 2009
DOI 10.1111/j.1469-8137.2009.03005.x

Fig. 3 GUS

Figure 3. The enhancer trap in line CS24365 disrupts the expression of AtrbohB (At1g09090). (a) Localisation of the insertion in enhancer trap line CS24365 insertion to chromosom 1, bp 2932468, in the promoter region of AtrbohB (At1g09090). (b) GUS staining of imbibed seeds leads to a strong blue colouring of the whole embryo. An isolated embryo is shown. (c) Semiquantitative RT-PCR comparing expression of AtrbohB transcripts in 24 h imbibed seeds of WT and the enhancer trap line CS24365. The AtrbohB transcript is only detected in the WT, but not in the mutant. 5.8S rRNA served as internal standard.


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Figure 6         Suppl. Figures S1&S2          Suppl. Table S1
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