Plant Physiology 161: 1903-1917 (2013)

Spatiotemporal seed development analysis provides insight into primary dormancy induction and evolution of the Lepidium DELAY OF GERMINATION1 Genes ^[W][OA]

School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (KG, AV, GLM);
Web: 'The Seed Biology Place' - www.seedbiology.eu
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (KG, AV, ABM, GLM)
Universität Osnabrück, Fachbereich Biologie, Botanik, D-49069 Osnabrück, Germany (KS, KM)

Received December 24, 2012; Accepted Feburary 19, 2013; Published Feburary 20, 2013.
DOI:10.1104/pp.112.213298

Lepidium species	GenBank accession number	Sequence type [Gene name used in text]	Size (bp)	Primer paird	Sequence similarity (%) to LesaDOG1a	Accession number	Origin
L. campestre	JX512181	genomic	1146	B	72.0	OSBU 96-0070-30-00	Greece, Thrace, Nomos Evrou, Korimvos
L. africanumc	JX512177	genomic	1323	B	66.3	OSBU 22265	Australia, Melbourne, Royal Park, south of Park Street; coll. N. Scarlett
L. pseudohyssopifoliumc	JX512179	genomic	1152	B	66.6	OSBU 22263	Australia Victoria, Mitre Rock, near Mt. Arapiles; 141°50`E 36°44`S;  coll. N. H. Scarlett
L. ruderalec	JX512180	genomic	1369	B	64.1	OSBU 05-0209-10-00	East Kazakhstan, Zaissan distr., Akzhan; coll. Antonyuk
L. bonariensec	JX512178	genomic	1238	B	71.8	OSBU 95-0022-10-00	Australia, NSW, Broken Hill; coll. H. Hurka
L. papillosum	JX512183	genomic [LepaDOG1a]	1343	Ae	85.0	Mummenhoff 2415	Australia, NSW, 10 km west of Broken Hill
L. papillosum	JX512187	mRNA [LepaDOG1a]	442	E	100.0	Mummenhoff 2415	dito
L. papillosum	JX512184	genomic [LepaDOG1b]	1317	Be	73.5	Mummenhoff 2415	dito
L. papillosum	JX512185	genomic [LepaDOG1c]	1583	Ce	65.6	Mummenhoff 2415	dito
L. papillosum	JX512188	mRNA [LepaDOG1c]	386	F	90.4	Mummenhoff 2415	dito
L. papillosum	JX512186	genomic [LepaDOG1d]	832	D	43.6	Mummenhoff 2415	dito
L. didymum	JX512176	genomic	1216	B	74.0	OSBU 22266	Germany, Osnabrück, near City Hall; coll. K. Sperber
L. leptopetalumb	JX512182	genomic	279	C	87.5	OSBU 22264	Australia, Victoria, near Carwarp; 142°06`E 34°38`S, coll. A.H. Brown

a Cloned genomic fragments have been aligned against genomic LesaDOG1 sequence; cloned cDNA sequences have been aligned against LesaDOG1 cDNA sequence. Similarity is the % pairwise identity within the aligned region. Global alignment was performed using MUSCLE. All genomic fragments cover at least partially exon 1 and exon 2 including the whole intron.
b This genomic fragment only covers exon 1.
c For these species also another smaller genomic fragment covering only exon 1 was cloned, which is not identical to the listed larger genomic fragment; this indicates the presence of multiple DOG1 genes in these species.
d Primer pair abbreviations refer to Supplemental Table S2.
e These primer pairs were used for the initial cloning of L. papillosum DOG1 gDNA fragments. To extend these sequences primers were designed based on an alignment of these gDNA sequences at highly divergent regions to obtain specific genomic fragments of the individual L. papillosum DOG1 genes. Consensus sequences of these genomic fragments were derived for each L. papillosum DOG1 gene.