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Journal of Experimental Botany 63: 5337-5350 (2012)

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' - www.seedbiology.eu
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Figure 4. The effect of MyA on the relationship between embryo size, growth and endosperm rupture at different ambient water potentials (Ψmedium).
(a) The percentages of endosperm rupture at different times during the seed germination of L. sativum at the various Ψmedium without (CON) and with 5x10-4 M MyA (Fig. 3) are plotted against the corresponding embryo mask areas. Note that while the onset of endosperm rupture was associated with an equal embryo size of 4.6-4.7 mm2, the progression of endosperm rupture required larger embryos in the MyA-series compared to CON; the embryo sizes required for 50% endosperm rupture (ER50%) were 5.4±0.1 mm2 (CON) and 6.8±0.2 mm2 (MyA), and therefore 1.3-fold upon MyA-treatment.
(b) Embryo sizes and endosperm rupture (upper panel), and embryo growth rates (lower panel; slopes of the curves in Fig. 3) at different Ψmedium during late germination. Mean values ±SE are presented from data shown in Figs. 3 and S3b.

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Supplementary data file (2 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Table S1

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Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]