Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' -
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Apoplastic ROS

Figure 7. Accumulation of apoplastic superoxide (O2•-) in Lepidium sativum embryos extracted from seeds incubated without (CON) or with 5x10-4 M MyA (MyA) in darkness.
(a) In situ localization of superoxide radical O2•- by NBT histostaining of embryos isolated from CON- and MyA-treated seeds incubated in darkness for 15h and 22h. Seeds without endosperm rupture (-ER) are presented for CON and MyA-treated seeds at 15h and 22h of incubation and with completed endosperm rupture (+ER) for CON at 22h. Left images show the lower, right images show the upper side of the embryos as positioned in the seed during incubation in the petri dish.
Quantification of apoplastic O2•- production using the XTT assay in (b) RAD (radicle+lower hypocotyl) and (c) CAP (micropylar endosperm) isolated from CON- and MyA-seeds incubated for 22h in darkness (this work) and for comparison in continuous light (Oracz et al. 2012). Mean values ± SE of four biological replicates.

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